Antioxidant and anti-inflammatory activities of the methanolic extract of Artemisia campestris L.

Abstract

Artemisia campestris, locally known as "T'gouft" and belonging to the Asteraceae family, has been traditionally used in algerian traditional medicine and as a condiment. Artemisia is recognized for its various bioactivities, including anti-inflammatory, antioxidant, and antimicrobial properties. This study aimed to assess the antioxidant and anti-inflammatory potential of Artemisia. The hydro-methanolic extract was analyzed through in vitro tests to evaluate phenolic content and antioxidant activity. Total phenolics and flavonoids were analyzed using Folin-Ciocalteu and aluminum chloride methods and the contents were154.8±2.74μgEqGA/mgand 34.59 ± 0.98μgEqQ/mg of extract, respectively. The antioxidant activity was evaluated using the DPPH assay, revealing a remarkable antioxidant property of the AC extract with an IC50 value of 120.04 ± 1.02μg/ml. Moreover, the methanolic extract showed a highly significant inhibition of paw edema induced by carrageenan in mice, comparable to diclofenac, with 200 and 400 mg/kg doses. These findings support the traditional use of A. campestris in treating inflammatory diseases, highlighting its appreciable anti-inflammatory and antioxidant activities.