Effets des modes de Laguerre Gauss sur la PSF microscopique

Abstract

Abstract In the world of optical microscopy, there are around ten methods for improving the spatial resolution of microscopic objectives. Namely, the use of immersion techniques, optimization through the application of de-convolution and many others. This end-of-study project aims to potentially improve the PSF of microscopic objectives by using higher order laser sources, namely Laguerre-Gauss modes. The latter are among the hot topics in the world of optics and applied photonics thanks to their remarkable properties in the fields of atom guidance, drilling and laser cutting and of course in optical microscopy. In parallel, a mathematical model based on the use of high-order Laguerre-Gauss sources will be adapted in order to see the effects of these laser modes on the PSF of the microscopic objective, in particular, the effect on the spatial resolution (axial and lateral). This can lead not only to better exploitation of the performance of the microscope, but also to the practical optimization of its resolution for various applications, notably in cellular and molecular biology, etc.