Study of anti-inflammatory and immunomodulatory properties of Ammodaucus leucotrichus

Abstract

This study investigated the potential of Ammodaucus leucotrichus seed extracts as a therapeutic agent for rheumatoid arthritis and joint inflammation.Methanol and n-hexane extracts were tested for their anti-inflammatory and anti-arthritic properties. The methanol extract demonstrated a significant ability to inhibit trypsin, and the n-hexane extract showed a remarkable ability to prevent the denaturation of bovine serum albumin.Rheumatoid arthritis was induced in rat models using two methods: immunization with Freund’s complete adjuvant and a solution of type II collagen derived from chicken cartilage.The therapeutic efficacy of the extracts was evaluated at varying doses and compared with methotrexate,a standard treatment for rheumatoid arthritis in humans.Both the extracts and methotrexate improved the mobility of the rats, as evidenced by increased walking distance, stride length, intra-step distance, and footprint area. They also positively influenced biochemical markers of inflammation. In silico docking studies identified several compounds with potential trypsin inhibitory activity: 2-hydroxyaceto hydrazide showing superior inhibitory effects compared to diclofenac. In conclusion, this study provides compelling evidence that suggests the potential of Ammodaucus leucotrichus seed