Gastropotective and antidiabetic effects of Phlomis crinita and Phlomis herbaventi extracts

Abstract

The present study was aimed to evaluate phenolic content,antioxidant and antidiabetic activity,and gastroprotective effect of methanolic(PC ME,PV ME)and aqueous extracts(PC AQE,PV AQE)of aerial parts of P.crinita and P.herba venti which are plants species of the Lamiaceae family.This study,showed that methanolic extract of P.herba venti contained high polyphenol and flavonoid contents,while the high level of tannins was registered in P.crinita methanolic extract. The antioxidant properties of plants extracts were determined using DPPH,ABTS,β-carotene bleaching,reducing power and metal chelating activity assays.P.herba venti extracts showed strong DPPH, ABTS radical scavenging activity and reducing power;while P.crinita extracts exhibited a good activity in β-carotene bleaching and metal chelating activity assays.After Induction of diabetes,the concentration of blood glucose increased in the alloxan group in comparison with normal ones,but the body weight decreased in the alloxan animals.The administration of PC ME and PV ME at doses of 250 and 500 mg/kg increased catalase activity,GSH level and decreased lipid peroxidation in the tissues of liver and kidneys,serum creatinin,uric acid,total cholesterol and triglycerides levels.In vitro inhibitory effect on diabetic enzyme showed that the methanolic extract of P.herba venti was inhibited(IC50 = 0.16±0.01 mg/mL)more than positive control on alphaglycosidase.Treatment of rats with 250 and 500 mg/kg of PC ME,PC AQE,PVME and PV AQE protected the stomach(68 to 95%)against ethanol(70%)induced ulcer and higher protection in comparison with ranitidine (positive control).This protection may be related to the augmentation of levels of total protein,GSH levels and/or inhibition of lipid peroxidation.