Efficacité d’une protéine entomotoxique du type a1b des graines de légumineuses

Abstract

Legume seeds are toxic to pests in stored grain. The effect of the protein PA1b pea (Pisum

sativum), chickpea (Cicer arietinum), bean (Phaseolus vulgaris), lentil (Lens esculenta) and bean

(Vicia fabae), was evaluated at concentrations of 10, 20, 40, 60 and 80%. Legume seeds are toxic to

pests in stored grain. The effect of the protein PA1b pea (Pisum sativum), chickpea (Cicer arietinum),

bean (Phaseolus vulgaris), lentil (Lens esculenta) and bean (Vicia fabae), was evaluated at

concentrations of 10, 20, 40, 60 and 80%. We note that all legumes tested are toxic to the species

Sitophilus granarius L. and a total mortality of the insect after 14 days. On 3

rd

day, there is in general

1-2 dead on wheat up to 15 days or the experiment was stopped. On peas, there is a mortality

exceeding 10% at a concentration of 80% of PA1b. From 7 days, there are no survivors. For

concentrations of 40% and 60%, mortality rates were 73.3% and 93.3% respectively; but, the rates are

low, not exceeding the threshold of 20% for concentrations of 20% and 10%. Some legume species

cause total mortality of the insect as well as 60% to 80%. This is the case of the lens, beans and bean.

Others are less toxic as chickpea which causes lower mortality of about 3 and 23% respectively.