Phytochemical,Antioxidant and Anti-inflammatory Effects of Medicinal Plants Extracts

Abstract

The aim of this study is to evaluate, in vitroand in vivo, the antioxidant, anti-inflammatoryand hepatoprotective activities of different extracts prepared from the aerial parts of Arthrophytum scopariumand Halogeton sativus. The contents of polyphenols, flavonoids and tannins of the crude methanolic extract (CrE) and its fractions: extract of chloroform (ChE), ethyl acetate (EAE), aqueous (EQA) and decoction (DEC) have been determined. The results showed that the DEC extract of A. scoparium contains the largest amount of phenolic compounds, flavonoids and tannins, whereas for H. sativus EAE contained the largest amount of polyphenols, flavonoids and tannins. CrE extracts from A. scopariumand EAE from H. sativusshowedthe highest scavenger (DPPH, ABTS and hydroxyl radicals), reductive and ion chelating activities. DECs of A. scopariumand H. sativusCrE exhibited the greatest activity of eliminating hydrogen peroxide. EAE extracts from H. sativusand CrE from A. scopariumwere found to be the most potent inhibitors of β-carotene oxidation with 99.12 ± 0.41% and 69.35 ± 0.32%, respectively. The DECs and CrEs of both plants showed the highest anti-hemolytic effect against AAPH-induced hemolysis, with significant HT50values. In addition, A. ScopariumCrE and H. sativusEAE have satisfactory inhibitory properties against FeSO4-induced hemolysis. In a hepatoprotective study, ASE (A.scopariumcrude extract) and HSE (H.sativuscrude extract) were administered orally (250 and 500 mg / kg) for 7 consecutive days and then, after 24 hours, CCl4was administered. The treatment of rats with CC14resulted in a significant increase in serum levels of ALT, AST, urea and creatinine. The analysis of the antioxidant potential parameters showed a significant increase in the GSH level and CAT activity of the groups treated with ASE and HSE. On the other hand, a significant decrease in MDA levels has been recorded. These parameters were significantly altered ina dose depending manner. In vivo, ASEand HSE showed a potent entrapment effect on DPPH and a reducing power. A histological study confirmed the antihepatotoxic potential of the extracts of both plants. Anti-inflammatory activities showed that the administration of 100, 200 and 400 mg / kg of ASEand HSE inhibited xylene-induced ear edema in a dose-dependent manner. The gastroprotective effect of ASEand HSE at a dose of 300 mg / kg was evaluated by restoring the architecture of the rats' stomachs. In conclusion, A. scopariumand H. sativusexert potent antioxidant activity both in vitroand in vivo. The selected plants can therefore be used as antioxidant, anti-inflammatory and hepatoprotective agents. The results of this study support the traditional use of these plants to treat many diseases.