Evaluation of Antioxidant and Antibacterial Properties of Peaganum Harmala Seed Extracts

Abstract

This study consist to evaluate the antioxidant and antibacterial activities of the phenolic fractions extracted from Peaganum harmala seeds by many organic solvents. The antioxidant activities of these extracts were studied by two complementary test; FRAP and NBT assay. The antibacterial effects were carried out using different bacterial strains; (E. coli ATCC 25922, Pseudomonas aeroginosa ATCC 27853, Staphylococus aureus ATCC 25923, Salmonella typhimurium ATCC 13311, Acinetobacter baumanii ATCC 19606, klebsiella pneumoniae ATCC 700603, Bacillus cereus ATCC10876, Enterococcus faecalis ATCC 49452 Lysteria monocytogenes ATCC 15313, Citrobacter freundii ATCC8090). The results indicate that the highest total polyphenols and flavonoids contents were recorded in chloroform extract (CHE) (66.29 mg gallic acid equivalent/mg of extract and 13.88 mg quercetin equivalent/mg of extract, respectively). In NBT assay CHE exhibited the best effect with an IC50 of 0,395mg/ml. The extracts exhibited a good reducing power, especially for CHE which gave the greatest reducing power (TAP = 10.422 mM Fe+2/g of extract). The antimicrobial activity was tested on 11 bacteria using the agar diffusion method. The ethyl acetate extract (EAE) have shown a significant antimicrobial activity against Gram (+) and Gram (-) bacteria.