Phytochemical and therapeutical valorisation of galium tunetanum poiret

Abstract

Galium tunetanum Poiret is one of dense pre-existent forest vestige belong to Rubiaceae is a North African endemic species constitute an enigma toward their phytochemicals and therapeutic proprieties. Areal part of the plant material is harvested from Megriss – Setif - Algeria, dried in shade, and reduced to a fine powder; then the major groups of secondary metabolites were extracted and quantified. The evaluation of antioxidant activities of species were carried out with DPPH, β-Carotene bleaching test and reducing power assay. Whereas, the antimicrobial capacity was tested against six bacterial strains, three fungi and one yeast. The anti-inflammatory activity was investigated in vitro and in vivo by human red blood cell (HRBC) membrane stabilization method, the denaturation of protein in vitro, Xylene-induced ear oedema in mice and cotton pellet-induced granuloma. The analgesic effect of the flavonoids extracted from Galium tunetanum Poiret was studied using hot plate and acetic acid induced writhing tests. The antiulcer activity of tannins were carry out by the preventive and curative test by studying various parameters. All of these therapeutic activities were completed by the acute toxicity study of methanolic extract. Polyphenols and saponins can be good antioxidant agents. However, they were less active or inactive against strains used in antimicrobial activity. Phenolic compounds demonstrate good capacities to be used against inflammatory diseases, especially the flavonoids that are nontoxic and efficacy against this kind of diseases at low doses. Those flavonoids have also potent analgesic activity and demonstrate the utility of these molecules on central mechanism of pain inhibition. Moreover, tannins provide protection against gastric mucosal damage induced by either ethanol/HCl or absolute ethanol, and the methanolic extract was found to possess significant wound healing activity .